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Arteriosclerosis, Thrombosis, and Vascular Biology current issue

Vascular Incorporation of Endothelial Colony-Forming Cells Is Essential for Functional Recovery of Murine Ischemic Tissue Following Cell Therapy [Integrative Biology/Experimental Medicine]
Schwarz, T. M., Leicht, S. F., Radic, T., Rodriguez-Araboalaza, I., Hermann, P. C., Berger, F., Saif, J., Bocker, W., Ellwart, J. W., Aicher, A., Heeschen, C. Wed, 18 Jan 2012 13:04:02 -0800
Objective— Cord blood–derived human endothelial colony-forming cells (ECFCs) bear a high proliferative capacity and potently enhance tissue neovascularization in vivo. Here, we investigated whether the leading mechanism for the functional improvement relates to their physical vascular incorporation or perivascular paracrine effects and whether the effects can be further enhanced by dual-cell–based therapy, including mesenchymal stem cells (MSCs). Methods and Results— ECFCs or MSCs were lentivirally transduced with thymidine kinase suicide gene driven by the endothelial-specific vascular endothelial growth factor 2 (kinase insert domain receptor) promoter and evaluated in a hindlimb ischemia model. ECFCs and MSCs enhanced neovascularization after ischemic events to a similar extent. Dual therapy using ECFCs and MSCs further enhanced neovascularization. Mechanistically, 3 weeks after induction of ischemia followed by cell therapy, ganciclovir-mediated elimination of kinase insert domain receptor+ cells completely reversed the therapeutic effect of ECFCs but not that of MSCs. Histological analysis revealed that ganciclovir effectively eliminated ECFCs incorporated into the vasculature. Conclusion— Endothelial-specific suicide gene technology demonstrates distinct mechanisms for ECFCs and MSCs, with complete abolishment of ECFC-mediated effects, whereas MSC-mediated effects remained unaffected. These data strengthen the notion that a dual-cell–based therapy represents a promising approach for vascular regeneration of ischemic tissue.
Targeting Angiogenesis as Treatment for Obesity [Editorial]
Bruemmer, D. Wed, 18 Jan 2012 13:04:00 -0800

Humanizing the Problem of Transplant Vasculopathy [Editorials]
Mendez-Fernandez, Y. V., Major, A. S. Wed, 18 Jan 2012 13:04:00 -0800

Endothelial-to-Mesenchymal Transition and MicroRNA-21: The Game Is On Again [Editorials]
Cavarretta, E., Latronico, M. V. G., Condorelli, G. Wed, 18 Jan 2012 13:04:00 -0800

Intraplaque Hemorrhage: An Imaging Marker for Atherosclerotic Plaque Destabilization? [Editorials]
Pasterkamp, G., van der Steen, A. F. W. Wed, 18 Jan 2012 13:04:00 -0800

Life After GWAS: Functional Genomics in Vascular Biology [ATVB in Focus: Life After GWAS: Functional Genomics in Vascular Biology]
Lusis, A. J. Wed, 18 Jan 2012 13:04:00 -0800


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